C7762
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 | α-Chymotrypsin is a serine protease of the peptidase S1 family consisting of 241 amino acid residues. The molecule has three peptide chains: an A chain of 13 residues, a B chain of 131 residues, and a C chain of 97 residues. α-Chymotrypsin is the predominant form of active enzyme produced from its zymogen, Chymotrypsinogen A. |
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C7762
Sigma
α-Chymotrypsin from bovine pancreas
Type I-S, essentially salt-free, lyophilized powder
| CAS Number: | 9004-07-3 |
| Enzyme Commission (EC) Number: | 3.4.21.1 ( BRENDA | IUBMB ) |
| EC Number: | 232-671-2 |
| MDL number: | MFCD00130481 |
Description
| Analysis Note | Minimum 85% protein |
| Protein determined by E | |
| Biochem/physiol Actions | A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. |
| Other Notes | View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer |
| Preparation Note | Prepared free of autolysis products and low molecular weight contaminants. |
| Derived from New Zealand-sourced pancreas | |
| Unit Definition | One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C. |
Properties
| type | Type I-S |
| form | essentially salt-free, lyophilized powder |
| activity | ≥40 units/mg protein |
| mol wt | mol wt 25 kDa |
| purified by | 3× crystallization |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| WGK Germany | 1 |
| RTECS | GC3050000 |
Related Products
| Substrate | A9273, Ala-Ala-Val-Ala p-nitroanilide |
| S8758, N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin | |
| S7388, N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide | |
| M4507, 4-Methylumbelliferyl p-trimethylammoniocinnamate chloride | |
| S9761, N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin | |
| G3769, Glutaryl-Ala-Ala-Phe-4-methoxy-β-naphthylamide | |
| S1899, N-Succinyl-Gly-Gly-Phe-p-nitroanilide | |
| A3401, Ala-Ala-Phe-7-amido-4-methylcoumarin | |
| G4636, N-Glutaryl-Gly-Gly-Phe-β-naphthylamide | |
| Inhibitor | A9285, α1-Antichymotrypsin from human plasma |
| D0879, Diisopropylfluorophosphate | |
| A1153, Aprotinin from bovine lung | |
| A2504, 6-Aminocaproic acid | |
| T1886, Trypsin inhibitor from chicken egg white | |
| E1398, Ecotin | |
| T9777, Trypsin-chymotrypsin inhibitor from Glycine max (soybean) | |
| T4376, N-p-Tosyl- | |
| B1552, Bromoenol lactone | |
| P7626, Phenylmethanesulfonyl fluoride | |
| A9024, α1-Antitrypsin from human plasma | |
| E7888, N-Acetyl-eglin C | |
| L2023, Leupeptin trifluoroacetate salt | |
| A6150, α1-Antitrypsin from human plasma | |
| A7824, 6-Aminocaproic acid | |
| C7268, Chymostatin |
References
| reference | Yapel, A., et al. J. Am. Chem. Soc. 88, 2573, (1966) |
| Merck | Merck 13,2282 |
